音時雨 ～Regentropfen～

There were some faults in this week, and all of them were about my experiments: my group members and I wanted extract chloroplasts.
That experiment was so tedious. We grinded 100g leaves with the terrible-smelled grind-liquid that included mercaptoethanol. After that, we did almost ten times centrifuge. When we have done our last centrifuge, I got out the centrifugal tube. We saw from outside.
There was nothing, no sediment but only the green-stained tube wall.
“Oh, nothing, that’s failed.”
We took half of morning and whole afternoon, but we got nothing.
Well, how can we finish out experiment report?

Now, see some good thing.
Last week I have done my experiment of Cell Engineering. For some technique support, we only did the part of plant culture. We every group used 40 conical beakers, divided into 8 groups for fill each group with one medium. In the eight kinds of medium, one we got to culture rapeseeds, the others we used for tobacco culturing. After one week, they sprouted out. Except few mold infected ones, I took some beautiful photographs. We were lucky, for many groups’ got almost fully mold infected. :eye:

Another thing I must mark here.
On Wednesday, I finished my drawing of the mini-story that I mentioned before. That was only a raw draft. I plan to get them into a series picture book, by mixture with backgrounds and caption texts. Well, my story is still Japanese only; I don’t know when I can translate them into English. However, I really think I am not good at colorful writing, so that even I myself don’t think this story will be colorful – never of Japanese version or English Version. What I did was only narrating.

During the 2 days, I was searching in GenBank for the genes I need. Nevertheless, when I input the key word “antifungal peptide,” GenBank gave me hundreds of matched results! Which I need are that produced by rape and radish and similar ones else. I can sure there is must some advanced search tool in GenBank, but I could not found it. I even got mad at the so much results for I was puzzled with how could I find out my need ones. Then I little regretted because I did not work hard on my Bioinformatics. Oh, dear! To hell with the endless search! I gave up my GenBank work. Then, maybe it was a gift from God, I found out some specific antimicrobial peptide databases! One of them, ANTIMIC, is very splendid! It is easy to search, and another, every search item has a link to GenBank or other database. It made me delighted.
Maybe there is something wrong of our college internet workgroup; maybe there are too much person are using the internet; maybe my laptop is so old that it can not still work quickly (well, of course, that is impossible. My laptop is all right though it produced in 2002). In all, the speed of my online working made me sick!

This term I have not so much class, so I decided that I ought to improve my English level from every aspect. Now I am doing listing and basic grammar.
In every morning, I do a piece of 9 minutes or so listing, one piece a day, and today is the nineteenth day. I listen and write what I listened down – the whole process often takes me about one and half hour. In the noon, I back to my dormitory, power up my laptop, check my written-down with the script. Sometimes I can get a good work, and sometimes it is terrible. Some words I did wrong but I indeed know it. I cannot identify them because of my Kana-like pronunciation (laugh); the English pronunciation style, such as “a,” “that” etc, are always diluted in a long sentence, also makes me fail to do well. Every time when I finished my listening, my right hand is so tired that even collapsed. Will, I indeed write too much, only by my single right hand, for my left hand cannot write smoothly.
In the afternoon, I take one or more hours to study grammar – that is also a work include much of note writing. So once again, my right hand turns to collapse.

Work in front of screen, write down what I listened, note each important item, and soon I will go into my lab (when I have found out all my needed gene sequences and designed out their matched primers). That is my latest day routine, not so colorful but should be fruitful.

Today is Halloween, but there is no any atmosphere. No matter, I wanted to do something at this day. Although I stay in America, I still cannot knock someone and say “trick or treat,” for I have not a child any more. Therefore, I decided to enjoy my own Halloween by me myself.
One of my friends and me went out this afternoon. She would go to bank before that. She got a waiting number and there were still 26 people waiting. In this time, I wanted to go to supermarket at first, and then back to meet her. She also wanted to go, and we thought when we back it was her turn; we went to supermarket together finally.
Maybe it was little strange, I bought six small cakes for my supper and my Halloween.
When we backed to bank again, the speaker said, “No.302, please.”
“Ah?! ” she was very surprised.
“What’s the matter?”
“Oh, dear! Mine is no.301…”
In all, we missed just only some seconds…
That time, I found God tricked us…
Well, however, later we went into a small candy shop and bought many lovely marshmallows and candies.
By then, all my Halloween preparations were OK!
This night would be happy, at least.

PS. I decided my nest picture theme should be “Halloween.”

ハロウィンという日なので、こちらはぜんぜん気分はない。でもやはり少しだけ何とかしよう。自分はもう子供じゃないし、アメリカでも他の人のドアを叩くわけがない。そうだったら、むしろ自分で祝おうと思った。
ということ、午後友達のみちゃんとお買うものに行った。その前、彼女は銀行に行くつもりだったのから、番号をとって見ると、まだ26人は前に待った。と、この待つ間、彼女を銀行に待たせて、私はスパーへ行くつもりだったけど、彼女も行きたかった。26人を待つ時間ならちょうどスパーに行く時間とぴったりはずだと思って、私たちはスパーに行った。
ちょっと変だったが、スパーにケーキを買ってハロウィンに用意した。
そしてもう一度銀行に変えてきたとき、拡声器は番号を放送しているところだった。
「No.302のお客様、ご受け付きください。」
「ありゃ…」と彼女が驚いた。
「どうしたの？」
「私の番号は301だもん…」
あはは…すれ違ったわね、つい先に…
ところで、こんなことは何だか、神様にトリックされたの様な気がする。
まあ、そのあと、私たちは一つ小さな店へ、かわいいマシマロやキャンディーをたくさん買った。
これでは、ハロウィンの準備は全部オーケー！
せめて今夜は楽しそうはずだわね！

追伸　私、決めてきた、次の絵のテーマはハロウィンなのです！